Bioconversion of Ajmalicine to Serpentine in Catharanthus roseus Roots
نویسندگان
چکیده
منابع مشابه
Screening of Catharanthus roseus secondary metabolites by high-performance liquid chromatography.
Two direct HPLC analytical methods for the screening of the major indole alkaloids of Catharanthus roseus hairy roots and their iridoid precursors have been developed. Photodiode array and fluorescence detection were performed. The separation was achieved on a reversed-phase C18 column. The first method allowed the separation of catharanthine, serpentine, tabersonine, vindoline, vinblastine, an...
متن کاملIndividual and Combined Effects of Azospirillum brasilense and Pseudomonas fluorescens on Biomass Yield and Ajmalicine Production in Catharanthus roseus
In the present study, the effect of different plant growth promoting rhizobacteria (PGPR) like Azospirillum brasilense and Pseudomonas fluorescens on growth parameters and the production of terpenoid indole alkaloids are investigated in two varieties ‘rosea’ and ‘alba’ of Catharanthus roseus. The production of IAA by the PGPR has taken as main criteria in the selection of PGPRs for the seed and...
متن کاملA simple and rapid HPLC-DAD method for simultaneously monitoring the accumulation of alkaloids and precursors in different parts and different developmental stages of Catharanthus roseus plants.
A rapid and simple reversed phase liquid chromatographic system has been developed for simultaneous analysis of terpenoid indole alkaloids (TIAs) and their precursors. This method allowed separation of 11 compounds consisting of eight TIAs (ajmalicine, serpentine, catharanthine, vindoline, vindolinine, vincristine, vinblastine, and anhydrovinblastine) and three related precursors i.e., tryptoph...
متن کاملThe transient effect of jasmonic acid feeding along with ORCA3 overexpression in Catharanthus roseus hairy roots
Jasmonic acid is an important signaling molecule in plants that regulates a wide variety of cellular responses including the responses to abiotic and biotic stresses. The external application of jasmonic acid has been shown to increase the accumulation of the monomeric terpenoid indole alkaloids, TIAs, in Catharanthus roseus seedlings and hairy root cultures (1-3). Jasmonic acid regulates the e...
متن کاملTranscriptome analysis in Catharanthus roseus leaves and roots for comparative terpenoid indole alkaloid profiles.
In Catharanthus roseus (L.) G. Don each tissue is known to produce a distinct spectrum of terpenoid indole alkaloids. Since the invaluable antineoplastic bisindole alkaloids are restricted to the aerial parts of the plant and do not occur in its underground tissues, identification of the structural and regulatory factors operating distinctly in the shoot/leaf of the plant will be a necessity fo...
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ژورنال
عنوان ژورنال: Journal of Plant Sciences
سال: 2006
ISSN: 1816-4951
DOI: 10.3923/jps.2006.340.347